DETECTION AND LOCALIZATION OF OSTEOCALCIN (BGP OR BONE GLA PROTEIN) IN TELEOSTS BY IN SITU HYBRIDATION AND IMMUNOHISTOCHEMISTRY

Paulo Gavaia; Carla SB Viegas; Pinto, JP; Sarasquete, MC; M. Leonor Cancela; Price, P A; Loureiro, Rui M. S.; Pena, Paula C. A.; M. Leonor Cancela; Cavaco, Sofia; Ferreira, Ana; Serra, Manuel; Theuwissen, Elke; Vermeer, Cees; D C Simes

Functional Biochemistry and Proteomics

Short Title

FBP

Description

The overall research strategy of FBP is driven primarily by the need to translate strong basic science into clinically relevant advances and promoting transfer of technology, based on the integration of high quality multi-disciplinary academic, researcher and clinical activity with a strong focus on translational research.

Coordinator

Dina Simes

Organiza

Main

The overall research strategy of FBP is driven primarily by the need to translate strong basic science into clinically relevant advances and promoting transfer of technology. FBP strategy is based on the integration of high quality multi-disciplinary academic, researcher and clinical activity with a strong focus on translational research.

FBP research team has long expertise in the biochemical aspects of vitamin K-dependent proteins (VKD) and aparticularly unique knowledge about the biochemistry, molecular biology and biomedical aspects of a new VKD member, Gla rich protein (GRP) for which methods and assays are proprietary IP (PCT/PT2009/000046).

Our research interests include the study of GRP and otherkey genes involved in processes of ectopic calcificationand inflammation related diseases such as atherosclerosis, cancer, osteoarthritis, renal disease and diabetes. Through a combination of in vivo studies using human biological samples and in vitro and ex vivo model systems, our research aims to establish new molecular knowledge on the pathophysiology and molecular mechanisms behind the onset and development of these highly prevalentdiseases. The output obtained will most certainly contribute to the prognostic, diagnostic and clinic therapeutics for pathological calcification and inflammation related diseases.

The research activities of FBP also include the exploitation of marine resources and the discovery and validation of natural products derived from marine organisms as novel pharmaceutical, health care and personal products to address key societal challenges. Novel drug leads are tested and developed using pre-clinical assays before clinical testing and translation into medical care or as potential and innovative cosmetics.

Main areas of research

The use of established pre-clinical human cell and tissue culture models, complemented with in vivo studies using a collection of well characterized human samples, for the study ofnovel intervening molecules and molecular mechanisms involved in highly prevalent diseases

Searching for novel disease biomarkers in circulation using collected human biological samples and correlation with clinical information.

Studies on the potential of circulating extracellular vesicles as novel biomarkers and drug delivery systems, with high diagnostic andtherapeutic value for calcification and inflammatory related diseases.
The use of in vitro and ex-vivo cell human modelsas pre-clinical assays for discovery and validationof marine bioactive products as novel diseasemodifying drugs (DMDs) and unveil theirmechanism of action.

Testing bioproducts from marine resources that could become potential and innovative cosmetics.

News

Nuna Araújo won the award for best oral communication abstract

PRESENÇA LUSA NO CONGRESSO ERA-EDTA

Novo biomarcador ajuda a detetar doenças cardiovasculares em doentes renais

European Perspectives in Cardiology European Perspectives in Cardiology: Cardiovascular research funded by the Portuguese Foundation for Science and Technology

Members

Enroll

Table view

Coordinator	Position
Dina Simes	Senior Researcher

Name	Position
Carla Alexandra São Bento Viegas	Research Assistant
Catarina Marreiros	Investigador
Dina Simes	Senior Researcher
Nuna Cláudia Peixoto de Araújo	PhD Student

Grid view

Carla Alexandra São Bento Viegas

Position:

Research Assistant

Catarina Marreiros

Position:

Investigador

Dina Simes

Position:

Senior Researcher

Nuna Cláudia Peixoto de Araújo

Position:

PhD Student

Former

Other Past Members

Sofia Cavaco

Marta Rafael

Ruben Costa

Lúcia Santos

Inês Perrolas

Sofia Santos

Inês Luis

Diego Gerini

Kevin Astorga

Gallery

Publications

All Publications


Viegas CSB, Simes DC. A dual role for GRP in cardiovascular disease. Aging. 2019;11(5):1323 - 1324. doi:10.18632/aging.v11i510.18632/aging.101851 Read more about A dual role for GRP in cardiovascular disease Google Scholar DOI
Simes DC, Viegas CSB, Araujo NC. Vitamin K as a Powerful Micronutrient in Aging and Age-Related Diseases: Pros and Cons from Clinical Studies. 2019. Read more about Vitamin K as a Powerful Micronutrient in Aging and Age-Related Diseases: Pros and Cons from Clinical Studies Google Scholar
The interplay between mineral metabolism, vascular calcification and inflammation in Chronic Kidney Disease (CKD): challenging old concepts with new facts. 2019. doi:10.18632/aging.102046 Read more about The interplay between mineral metabolism, vascular calcification and inflammation in Chronic Kidney Disease (CKD): challenging old concepts with new facts Google Scholar DOI
Viegas C, Edelweiss E, Schneider J, et al. Use of an innovative system and nanotechnology-based strategy for therapeutic applications of Gla-rich protein (GRP). Annals of Medicine. 2019;51(sup1):38 - 38. doi:10.1080/07853890.2018.1561804 Read more about Use of an innovative system and nanotechnology-based strategy for therapeutic applications of Gla-rich protein (GRP) Google Scholar DOI
Araújo N, Viegas C, Perrolas I, et al. Amentadione is a new modulating agent for osteoarthritis in an ex-vivo co-culture preclinical assay. Annals of Medicine. 2019;51(sup1):43 - 43. doi:10.1080/07853890.2018.1561895 Read more about Amentadione is a new modulating agent for osteoarthritis in an ex-vivo co-culture preclinical assay Google Scholar DOI
Viegas CSB, Macedo AL, Matos AA, et al. Translational Research and Innovation in Human and Health Science: Gla-rich protein, a vitamin K-dependent protein involved in inflammation and calcification-related diseases. Annals of Medicine. 2018;50(sup1):S1 - S9. doi:10.1080/07853890.2018.1427452 Read more about Translational Research and Innovation in Human and Health Science: Gla-rich protein, a vitamin K-dependent protein involved in inflammation and calcification-related diseases. Google Scholar DOI
Willems BA, Furmanik M, Caron MMJ, et al. Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling. Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-23353-y Read more about Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling Google Scholar DOI
Viegas CSB, Santos L, Macedo AL, et al. Chronic Kidney Disease Circulating Calciprotein Particles and Extracellular Vesicles Promote Vascular Calcification. Arteriosclerosis, Thrombosis, and Vascular Biology. 2018;38(3):575 - 587. doi:10.1161/ATVBAHA.117.310578 Read more about Chronic Kidney Disease Circulating Calciprotein Particles and Extracellular Vesicles Promote Vascular Calcification Google Scholar DOI
Silva APaula, Viegas C, Simes D, et al. GLA-RICH PROTEIN AS A NOVEL MARKER FOR CALCIFICATIONS IN DIABETIC PATIENTS WITH CKD. Nephrology Dialysis Transplantation. 2018;33(suppl_1):i493 - i493. doi:10.1093/ndt/gfy104.SP430 Read more about GLA-RICH PROTEIN AS A NOVEL MARKER FOR CALCIFICATIONS IN DIABETIC PATIENTS WITH CKD Google Scholar DOI
Viegas CSB, Simes DC. Immunity And Inflammation In Health And Disease: Inflammation And Calcification In The Vascular Tree; Insights Into Atherosclerosis. Elsevier; 2018:189 - 201. doi:10.1016/B978-0-12-805417-8.00015-9 Read more about Immunity and Inflammation in Health and Disease: Inflammation and Calcification in the Vascular Tree; Insights Into Atherosclerosis Google Scholar DOI
Viegas CSB, Costa RM, Santos L, et al. Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases. . de Frutos PGarcia, ed. PLOS ONE. 2017;12(5):e0177829. Read more about Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases. Google Scholar DOI
Viegas CSB, Macedo AL, Morais R, et al. Dysregulated fetuin–mineral complexes are linked to vascular calcification in chronic kidney disease: The role of Gla-rich protein. Ultrastructural Pathology. 2017;41(1):78 - 80. doi:10.1080/01913123.2016.1269490 Read more about Dysregulated fetuin–mineral complexes are linked to vascular calcification in chronic kidney disease: The role of Gla-rich protein Google Scholar DOI
Morais R, Viegas CSB, Simes DC, Matos APA, Macedo AL. Application of TEM techniques for the study of vascular calcification: Monitoring extracellular vesicles and nanogold immunodetection of fetuin-A, GRP, and CD9. Ultrastructural Pathology. 2017;41(1):130 - 132. doi:10.1080/01913123.2016.1272670 Read more about Application of TEM techniques for the study of vascular calcification: Monitoring extracellular vesicles and nanogold immunodetection of fetuin-A, GRP, and CD9 Google Scholar DOI
Viegas C, Santos L, Macedo A, et al. CIRCULATING CALCIPROTEIN PARTICLES AND EXTRACELLULAR VESICLES AS NOVEL PLAYERS IN CHRONIC KIDNEY DISEASE VASCULAR CALCIFICATION. A ROLE FOR GLA-RICH PROTEIN. Nephrology Dialysis Transplantation. 2017;32(suppl_3):iii67 - iii68. doi:10.1093/ndt/gfx123.MO055 Read more about CIRCULATING CALCIPROTEIN PARTICLES AND EXTRACELLULAR VESICLES AS NOVEL PLAYERS IN CHRONIC KIDNEY DISEASE VASCULAR CALCIFICATION. A ROLE FOR GLA-RICH PROTEIN Google Scholar DOI
Macedo AL, Viegas CSB, Matos APA, Simes DC. Extracellular Vesicles and Circulating Fetuin-Mineral Complexes in Vascular Calcification; Studies by EM Techniques. Microscopy and Microanalysis. 2016;22(S4):16 - 17. doi:10.1017/S1431927616000271 Read more about Extracellular Vesicles and Circulating Fetuin-Mineral Complexes in Vascular Calcification; Studies by EM Techniques Google Scholar DOI
Viegas CSB, Simes DC. New Perspectives for the Nutritional Value of Vitamin K in Human Health. Journal of Nutritional Disorders & Therapy. 2016;6(3). doi:10.4172/2161-050910.4172/2161-0509.1000192 Read more about New Perspectives for the Nutritional Value of Vitamin K in Human Health Google Scholar DOI
Viegas CSB, Simes DC. Gla-rich Protein (GRP): A New Player In The Burden Of Vascular Calcification. Journal of Cardiovascular Diseases & Diagnosis. 2016;4(4). doi:10.4172/2329-951710.4172/2329-9517.1000245 Read more about Gla-rich Protein (GRP): A New Player In The Burden Of Vascular Calcification Google Scholar DOI
Cavaco S, Viegas CSB, Rafael MS, et al. Gla-rich protein is involved in the cross-talk between calcification and inflammation in osteoarthritis. Cell Mol Life Sci. 2016;73(5):1051-65. doi:10.1007/s00018-015-2033-9 Read more about Gla-rich protein is involved in the cross-talk between calcification and inflammation in osteoarthritis. Google Scholar PubMed DOI
Viegas CSB, Rafael MS, Enriquez JL, et al. Gla-rich protein acts as a calcification inhibitor in the human cardiovascular system. Arterioscler Thromb Vasc Biol. 2015;35(2):399-408. doi:10.1161/ATVBAHA.114.304823 Read more about Gla-rich protein acts as a calcification inhibitor in the human cardiovascular system. Google Scholar PubMed DOI
Richard N, Engrola S, Palma PS, Simes DC, Conceição LEC. Assessment of protein digestive capacity and metabolic utilisation during ontogeny of Senegalese sole larvae: A tracer study using in vivo produced radiolabelled polypeptide fractions. Aquaculture. 2015;441:35 - 44. doi:10.1016/j.aquaculture.2015.02.003 Read more about Assessment of protein digestive capacity and metabolic utilisation during ontogeny of Senegalese sole larvae: A tracer study using in vivo produced radiolabelled polypeptide fractions Google Scholar DOI
Viegas CSB, Rafael MS, Enriquez JL, et al. Gla-rich protein (GRP) is a new player in mineralization-competence of extracellular vesicles involved in vascular calcification. Journal of Vascular Research. 2015;52(1):1 - 88. doi:10.1159/000433498 Read more about Gla-rich protein (GRP) is a new player in mineralization-competence of extracellular vesicles involved in vascular calcification Google Scholar DOI
Cavaco S, Williamson MK, Rosa J, et al. Teleost fish osteocalcin 1 and 2 share the ability to bind the calcium mineral phase. Fish Physiol Biochem. 2014;40(3):731-8. doi:10.1007/s10695-013-9880-9 Read more about Teleost fish osteocalcin 1 and 2 share the ability to bind the calcium mineral phase. Google Scholar PubMed DOI
Rafael MS, Cavaco S, Viegas CSB, et al. Insights into the association of Gla-rich protein and osteoarthritis, novel splice variants and γ-carboxylation status. Mol Nutr Food Res. 2014;58(8):1636-46. doi:10.1002/mnfr.201300941 Read more about Insights into the association of Gla-rich protein and osteoarthritis, novel splice variants and γ-carboxylation status. Google Scholar PubMed DOI
Viegas CSB, Herfs M, Rafael MS, et al. Gla-rich protein is a potential new vitamin K target in cancer: evidences for a direct GRP-mineral interaction. Biomed Res Int. 2014;2014:340216. doi:10.1155/2014/340216 Read more about Gla-rich protein is a potential new vitamin K target in cancer: evidences for a direct GRP-mineral interaction. Google Scholar PubMed DOI
Viegas CSB, Simes DC, Williamson MK, et al. Sturgeon osteocalcin shares structural features with matrix Gla protein: evolutionary relationship and functional implications. J Biol Chem. 2013;288(39):27801-11. doi:10.1074/jbc.M113.450213 Read more about Sturgeon osteocalcin shares structural features with matrix Gla protein: evolutionary relationship and functional implications. Google Scholar PubMed DOI
Oliveira DV, Silva TS, Cordeiro OD, Cavaco SI, Simes DC. Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from Crassostrea gigas nacre using a proteomic approach. ScientificWorldJournal. 2012;2012:765909. doi:10.1100/2012/765909 Read more about Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from Crassostrea gigas nacre using a proteomic approach. Google Scholar PubMed DOI
Viegas CSB, Conceição N, Fazenda C, Simes DC, M. Cancela L. Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon ( Acipenser naccarii ). Journal of Applied Ichthyology. 2010;26(2):214 - 218. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01408.x Read more about Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon ( Acipenser naccarii ) Google Scholar DOI
Roberto VP, Cavaco S, Viegas CSB, et al. Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation. Aquaculture. 2009;294(3-4):202 - 211. doi:10.1016/j.aquaculture.2009.06.020 Read more about Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation Google Scholar DOI
Viegas CSB, Cavaco S, Neves PL, et al. Gla-rich protein is a novel vitamin K-dependent protein present in serum that accumulates at sites of pathological calcifications. Am J Pathol. 2009;175(6):2288-98. doi:10.2353/ajpath.2009.090474 Read more about Gla-rich protein is a novel vitamin K-dependent protein present in serum that accumulates at sites of pathological calcifications. Google Scholar PubMed DOI
Viegas CSB, Simes DC, Laizé V, Williamson MK, Price PA, M. Cancela L. Gla-rich protein (GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly conserved in vertebrates. J Biol Chem. 2008;283(52):36655-64. doi:10.1074/jbc.M802761200 Read more about Gla-rich protein (GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly conserved in vertebrates. Google Scholar PubMed DOI
Rønnestad I, Gavaia P, Viegas CSB, et al. Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects. J Exp Biol. 2007;210(Pt 22):3883-96. doi:10.1242/jeb.007898 Read more about Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects. Google Scholar PubMed DOI
Simões B, Conceição N, Viegas CSB, et al. Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. Calcif Tissue Int. 2006;79(4):230-44. doi:10.1007/s00223-006-0111-6 Read more about Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. Google Scholar PubMed DOI
Gavaia P, Simes DC, Ortiz-Delgado JB, et al. Osteocalcin and matrix Gla protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): comparative gene and protein expression during larval development through adulthood. Gene Expr Patterns. 2006;6(6):637-52. doi:10.1016/j.modgep.2005.11.010 Read more about Osteocalcin and matrix Gla protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): comparative gene and protein expression during larval development through adulthood. Google Scholar PubMed DOI
Ortiz-Delgado JB, Simes DC, Viegas CSB, Schaff BJ, Sarasquete C, M. Cancela L. Cloning of matrix Gla protein in a marine cartilaginous fish, Prionace glauca: preferential protein accumulation in skeletal and vascular systems. Histochem Cell Biol. 2006;126(1):89-101. doi:10.1007/s00418-005-0125-6 Read more about Cloning of matrix Gla protein in a marine cartilaginous fish, Prionace glauca: preferential protein accumulation in skeletal and vascular systems. Google Scholar PubMed DOI
Laizé V, Viegas CSB, Price PA, M. Cancela L. Identification of an osteocalcin isoform in fish with a large acidic prodomain. Journal of Biological Chemistry. 2006;281(22):15037-15043. doi:10.1074/jbc.M600373200 Read more about Identification of an osteocalcin isoform in fish with a large acidic prodomain Google Scholar DOI
Laizé V, Martel P, Viegas CSB, Price PA, M. Cancela L. Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates. J Biol Chem. 2005;280(29):26659-68. doi:10.1074/jbc.M500257200 Read more about Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates. Google Scholar PubMed DOI
Ortiz-Delgado JB, Simes DC, Gavaia P, Sarasquete C, M. Cancela L. Osteocalcin and matrix GLA protein in developing teleost teeth: identification of sites of mRNA and protein accumulation at single cell resolution. Histochem Cell Biol. 2005;124(2):123-30. doi:10.1007/s00418-005-0015-y Read more about Osteocalcin and matrix GLA protein in developing teleost teeth: identification of sites of mRNA and protein accumulation at single cell resolution. Google Scholar PubMed DOI
Pinto JP, Conceição NM, Viegas CSB, et al. Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. J Bone Miner Res. 2005;20(8):1440-53. doi:10.1359/JBMR.050318 Read more about Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. Google Scholar PubMed DOI
Frazão C, Simes DC, Coelho R, et al. Structural evidence of a fourth Gla residue in fish osteocalcin: biological implications. Biochemistry. 2005;44(4):1234-42. doi:10.1021/bi048336z Read more about Structural evidence of a fourth Gla residue in fish osteocalcin: biological implications. Google Scholar PubMed DOI
Pinto JP, Conceição NM, Viegas CSB, et al. Identification of a New pebp2αA2 Isoform From Zebrafish runx2 Capable of Inducing Osteocalcin Gene Expression In Vitro. Journal of Bone and Mineral Research. 2005;20(8):1440 - 1453. doi:10.1359/JBMR.050318 Read more about Identification of a New pebp2αA2 Isoform From Zebrafish runx2 Capable of Inducing Osteocalcin Gene Expression In Vitro Google Scholar DOI
Laizé V, Martel P, Viegas CSB, Price PA, M. Cancela L. Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates. Journal of Biological Chemistry. 2005;280(29):26659-26668. doi:10.1074/jbc.M500257200 Read more about Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates Google Scholar DOI
Simes DC, Williamson MK, Schaff BJ, et al. Characterization of osteocalcin (BGP) and matrix Gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebrates. Calcif Tissue Int. 2004;74(2):170-80. doi:10.1007/s00223-003-0079-4 Read more about Characterization of osteocalcin (BGP) and matrix Gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebrates. Google Scholar PubMed DOI
Gavaia P, Ortiz JB, Simes DC, Sarasquete MC, M. Cancela L. SIMULTANEOUS DETECTION OF OSTEOCALCIN AND MATRIX GLA PROTEIN IN DEVELOPMENTAL STAGES OF ZEBRA FISH (DANIO RERIO). Calcified Tissue International. 2003;72:314 - 468. doi:10.1007/s00223-003-5002-5 Read more about SIMULTANEOUS DETECTION OF OSTEOCALCIN AND MATRIX GLA PROTEIN IN DEVELOPMENTAL STAGES OF ZEBRA FISH (DANIO RERIO) Google Scholar DOI
Simes DC, Williamson MK, Ortiz-Delgado JB, Viegas CSB, Price PA, M. Cancela L. Purification of matrix Gla protein from a marine teleost fish, Argyrosomus regius: calcified cartilage and not bone as the primary site of MGP accumulation in fish. J Bone Miner Res. 2003;18(2):244-59. doi:10.1359/jbmr.2003.18.2.244 Read more about Purification of matrix Gla protein from a marine teleost fish, Argyrosomus regius: calcified cartilage and not bone as the primary site of MGP accumulation in fish. Google Scholar PubMed DOI
Viegas CSB, Pinto JP, Conceição N, Simes DC, M. Cancela L. Cloning and characterization of the cDNA and gene encoding Xenopus laevis osteocalcin. Gene. 2002;289(1-2):97-107. Read more about Cloning and characterization of the cDNA and gene encoding Xenopus laevis osteocalcin. Google Scholar PubMed
Araújo NCP, Barroca PMM, Bickley JF, et al. Structural effects on sigmatropic shifts in heteroaromatic allyl ethersElectronic supplementary information (ESI) available: selected crystal data for compound 7. See http://www.rsc.org/suppdata/p1/b1/b102674g/. Journal of the Chemical Society, Perkin Transactions 1. 2002;(9):1213 - 1219. doi:10.1039/b102674g Read more about Structural effects on sigmatropic shifts in heteroaromatic allyl ethersElectronic supplementary information (ESI) available: selected crystal data for compound 7. See http://www.rsc.org/suppdata/p1/b1/b102674g/ Google Scholar DOI
M. Cancela L, Ohresser MC, Reia JP, Viegas CSB, Williamson MK, Price PA. Matrix Gla protein in Xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations. J Bone Miner Res. 2001;16(9):1611-21. doi:10.1359/jbmr.2001.16.9.1611 Read more about Matrix Gla protein in Xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations. Google Scholar PubMed DOI
Gavaia P, Viegas CSB, Pinto JP, Sarasquete MC, M. Cancela L. DETECTION AND LOCALIZATION OF OSTEOCALCIN (BGP OR BONE GLA PROTEIN) IN TELEOSTS BY IN SITU HYBRIDATION AND IMMUNOHISTOCHEMISTRY. Bone. 2001;28:S101 - S248. doi:10.1016/S8756-3282(01)00454-9 Read more about DETECTION AND LOCALIZATION OF OSTEOCALCIN (BGP OR BONE GLA PROTEIN) IN TELEOSTS BY IN SITU HYBRIDATION AND IMMUNOHISTOCHEMISTRY Google Scholar DOI

Projects

	Title	Coordinator	Time frame	Funding	CCMAR	All
	Gla-rich protein (GRP) associated to circulating calciprotein particles (CPP) and extracellular vesicles (EVs) as a novel marker of vascular calcification stress in diabetic with chronic kidney disease (CKD)	Carla Alexandra São Bento Viegas, Dina Simes	12/31/2019	Sociedade Portuguesa de Nefrologia	15.00k€
	Characterization of bioengineered extracellular vesicles (EVs) loaded with Gla-rich protein: a proteomic approach	Carla Alexandra São Bento Viegas, Dina Simes		EPIC-XS

	Title	Coordinator	Time frame	Funding	CCMAR
PROGLAGRP	Novos conhecimentos sobre o mecanismo de calcificação vascular em doença renal crónica (DRC): o papel da GRP	Carla Alexandra São Bento Viegas	05/01/2013 to 12/31/2015	FCT	150.18k€
BioGlaGRP	Elucidação da função e mecanismo de acção molecular da proteína Gla-rich (GRP) na calcificação vascular (BioGlaGRP)	Carla Alexandra São Bento Viegas	02/01/2012 to 09/30/2015	FCT	159.75k€
OSTEOARTGRP	Nova abordagem para a identificação de alvos terapeuticos da osteoartrite: aplicação da proteomica 2D-DIGE.	Dina Simes	01/01/2011 to 06/30/2014	FCT	150.00k€
BIOMARKGRP	Estudos conducentes ao establecimento da GRP como biomarcador da calcificação vascular	Dina Simes	01/01/2010 to 07/31/2013	FCT	117.74k€
POCI 57921	Função na MGP na regulação local da mineralização da matriz extracelular- Identificação dos motivos estruturais responsáveis por essa função.	Dina Simes	09/01/2005 to 06/30/2008	FCT	86.27k€

Events

Type of Event

Seminar

Unveiling the dual role of Gla-rich protein (GRP) in the crosstalk between inflammation and calcification in pathological processes

Carla Viegas will join us on 28 th of June to talk about "Unveiling the dual role of Gla-rich protein (GRP) in the crosstalk between inflammation and calcification in pathological processes". Don't...

June 28, 2017

Anf. B (CP) - Gambelas Campus UAlg

News

Nuna Araújo won the award for best oral communication abstract

This abstract is very relevant for the development of an innovating pre-clinical model test to select the best leads for OA (Osteoarthritis) therapeutics.

PRESENÇA LUSA NO CONGRESSO ERA-EDTA

INVESTIGAÇÃO LUSA A «DAR CARTAS» A 54.ª edição do Congresso da ERA-EDTA (European Renal Association-European Dialysis and Transplant Association), que teve lugar em Madrid, de 3 a 6 de junho 2017, contou com a intervenção de...

Novo biomarcador ajuda a detetar doenças cardiovasculares em doentes renais

Um grupo de investigadores do Centro de Ciências do Mar (CCMAR), em parceria com o Centro Hospitalar Universitário do Algarve e em conjunto com a GenoGla Diagnostics , desenvolveu um novo teste diagnóstico utilizando um...

European Perspectives in Cardiology European Perspectives in Cardiology: Cardiovascular research funded by the Portuguese Foundation for Science and Technology

Carla Alexandra São Bento Viegas, PhD, postdoctoral researcher, Functional Biochemistry and Proteomics group headed by Professor Dina Simes, PhD, Center of Marine Sciences, University of Algarve, Faro