PUBLICATIONS IN INTERNATIONAL PEER REVIEWED JOURNALS
Simes DC, Viegas CSB, Araújo N, Marreiros C. (2019) Vitamin K as a Powerful Micronutrient in Aging and Age-Related Diseases: Pros and Cons from Clinical Studies. Int J Mol Sci. 20(17). pii: E4150. doi: 10.3390/ijms20174150.
Viegas C, Araújo N, Marreiros C, Simes D. (2019) The interplay between mineral metabolism, vascular calcification and inflammation in Chronic Kidney Disease (CKD): challenging old concepts with new facts. Aging (Albany NY). 11(12):4274-4299.
Viegas CS, Edelweiss E, Schneider J, Schaeffer-Reiss C, Poterszman A, Rafael M, Araújo N, Macedo A, Matos AP & Simes DC. (2019) Use of an innovative system and nanotechnology-based strategy for therapeutic applications of Gla-rich protein (GRP), Annals of Medicine, 51:sup1, 38, DOI: 10.1080/07853890.2018.1561804
Araújo N, Viegas CS, Perrolas I, Costa R, Magalhães J, Blanco F, Ramos A, Miguel M, Vermeer C, Zubía E & Simes DC (2019) Amentadione is a new modulating agent for osteoarthritis in an ex-vivo co-culture preclinical assay, Annals of Medicine, 51:sup1, 43, DOI: 10.1080/07853890.2018.1561895
Viegas CSB, Simes DC. (2019) A dual role for GRP in cardiovascular diseases. Aging (Albany NY). 11(5):1323-1324. doi: 10.18632/aging.101851.
Viegas CSB, Macedo AL, Matos AA, Silva AP, Neves PL, Vermeer C, Schurgers L, Simes DC. (2018) Gla-rich protein, a vitamin K-dependent protein involved in inflammation and calcification-related diseases. Annals of Medicine, 50:sup1, S1-S9, DOI: 10.1080/07853890.2018.1427452 https://doi.org/10.1080/07853890.2018.1427452
Brecht A Willems, Malgorzata Furmanik, Marjolein MJ Caron, Martijn LL Chatrou, Dennis HM Kusters, Tim JM Welting, Michael Stock, Marta S Rafael, Carla SB Viegas, Dina C Simes, Cees Vermeer, Chris PM Reutelingsperger, Leon J Schurgers. (2018) Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling. Sci Rep. 21;8(1):4961. doi: 10.1038/s41598-018-23353-y.
Viegas CSB, Santos L, Macedo AL, Matos AP, Silva AP, Neves PL, Staes A, Gevaert K, Morais R, Vermeer C, Schurgers L, Simes DC. (2018) Chronic kidney disease circulating calciprotein particles and extracellular vesicles promote vascular calcification: a role for GRP (Gla-rich protein). Arterioscler Thromb Vasc Biol. 38(3):575-587. doi: 10.1161/ATVBAHA.117.310578.
Viegas CSB, Costa RM, Santos L, Videira PA, Silva Z, Araújo N, Macedo AL, Matos AP, Vermeer C, Simes DC. (2017) Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases. PLoS ONE 12(5): e0177829. https://doi.org/10.1371/journal.pone.0177829.
Viegas CS, Macedo AL, Morais R, Santos L, Matos AP, Silva AP, Neves P, Simes DC. (2017) Dysregulated Fetuin–Mineral Complexes are Linked to Vascular Calcification in Chronic Kidney Disease: The Role of Gla-Rich Protein. Ultrastructural Pathology, 41:1, 78-80, DOI: 10.1080/01913123.2016.1269490.
Morais R, Viegas CSB, Simes DC, Matos AP, Macedo AL. (2017) Application of TEM techniques for the study of vascular calcification: Monitoring extracellular vesicles and nanogold immunodetection of Fetuin-A, GRP, and CD9. Ultrastructural Pathology, 41:1, 130-132, DOI: 10.1080/01913123.2016.1272670.
Macedo AL, Viegas CSB, Matos APA, Simes DC. (2016) Extracellular Vesicles and Circulating Fetuin-Mineral Complexes in Vascular Calcification; Studies by EM Techniques. Microscopy and Microanalysis 22(S4):16-17. DOI: 10.1017/S1431927616000271
Viegas CS, Simes DC (2016). New Perspectives for the Nutritional Value of Vitamin K in Human Health. J Nutr Disorders Ther 2016, 6: 192. doi:10.4172/2161-0509.1000192
Viegas CS, Simes DC (2016). Gla-rich Protein (GRP): A New Player in the Burden of Vascular Calcification. J Cardiovasc Dis Diagn 4: 245. doi:10.4172/2329-9517.1000245
Cavaco S, Viegas CS, Rafael MS, Ramos A, Magalhães J, Blanco FJ, Vermeer C, Simes DC (2015). Gla-rich protein is involved in the cross-talk between calcification and inflammation in osteoarthritis. Cell Mol Life Sci. 73:1051-1065.
Viegas CS, Rafael MS, Enriquez JL, Teixeira A, Vitorino R, Luís IM, Costa RM, Santos S, Cavaco S, Neves J, Macedo AL, Willems BA, Vermeer C, Simes DC (2015). Gla-rich protein acts as a calcification inhibitor in the human cardiovascular system. Arterioscler Thromb Vasc Biol. 2015 Feb;35(2):399-408.
Rafael MS, Cavaco S, Viegas CS, Santos S, Ramos A, Willems B, Herfs M, Theuwissen E, Vermeer C, Simes DC (2014). Insights into the association of Gla-rich protein (GRP) and osteoarthritis: novel splice variants and ?-carboxylation status. Mol Nutr Food Res. 58(8):1636-46. doi: 10.1002/mnfr.201300941.
Viegas CS, Marjolein Herfs,Marta S Rafael, José L Enriquez, Alexandra Teixeira, Vera L Maria, Alexandre João, Sofia Cavaco,Ana Ferreira, Manuel Serra, ElkeTheuwissen, Cees Vermeer, Dina C Simes (2014). Gla-rich protein is a potential new vitamin K target in cancer: Evidences for a direct GRP-mineral interaction. Biomed Res Int. 2014:340216. doi:10.1155/2014/340216.
Ana Paula Silva, André Fragoso, Cláudia Silva, Carla SB Viegas, Nelson Tavares, Patrícia Guilherme, Nélio Santos, Fátima Rato, Ana Camacho, Cidália Cavaco, Victor Pereira, Marilia Faísca, João Ataíde, Ilídio Jesus and Pedro Leão Neves (2013). What is the role of apelin regarding cardiovascular risk and progression of renal disease in type 2 diabetic patients with diabetic nephropathy?. Biomed Res Int. 2013:247649. doi: 10.1155/2013/247649.
Viegas CS, Simes DC, Williamson MK, Cavaco S, Laize V, Price PA, Cancela ML (2013) Sturgeon Osteocalcin Shares Structural Features with Matrix Gla Protein: Evolutionary Relationship and Functional Implications. J Biol Chem. 288(39):27801-11. doi: 10.1074/jbc.M113.450213.
Viegas CS, Conceição N, Fazenda C, Simes DC, Cancela ML. (2010) Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon (Acipenser naccarii). J Appl.Ichthyol. 26: 214–218.
Viegas CS, Cavaco S, Neves P, Ferreira A, João A, Williamson MK, Price PA, Cancela ML, Simes D. (2009) Gla-rich protein (GRP) is a novel vitamin K dependent protein present in serum and accumulated at sites of pathological calcifications. Am J Pathol. Dec;175(6):2288-98. Epub 2009 Nov 5.
Le Corguille G, Pearson GA, Valente M, Viegas C, Gschloessl B, Corre E, Bailly X, Peters A, Jubin C, Vacherie B, Cock M, Leblanc C (2009); Chloroplast genomes of two brown algae, Ectocarpus siliculosus and Fucus vesiculosus: further insights on the evolution of red-algal derived plastids. BMC Evol Biol. Oct 16;9:253.
Roberto VP, Cavaco S, Viegas CS, Simes DC, Ortiz-Delgado JB, Sarasquete MC, Gavaia PJ and Cancela ML. (2009) Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation. Aquaculture Sept;294(3-4): 202-211.
Viegas CS, Simes D, Laize V, Williamson MK, Price PA, Cancela ML (2008) Gla-rich protein (GRP): a new vitamin-K dependent protein identified from sturgeon cartilage and highly conserved in vertebrates. J Biol Chem, Dec 26;283(52):36655.
Ronnestad I, Gavaia PJ, Viegas CS, Verri T, Romano A, Nilsen TO, Jordal AE, Kamisaka Y, Cancela ML. (2007) Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects. J Exp Biol. Nov; 210(22):3883-3896.
Simões B, Conceição N, Viegas CS, Pinto JP, Gavaia P, Kelsh RN and Cancela ML. (2006) Molecular cloning of the zebrafish (Danio rerio) Collagen Xa1 cDNA and gene. Studies on the developmental expression and identification of promoter regulatory elements. Calcif Tissue Int. 79(4):230-244.
Gavaia P, Simes D, Ortiz Delgado JB, Viegas CS, Pinto J, Kelsh RN, Sarasquete MC, Cancela ML. (2006) Osteocalcin and matrix Gla protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): Comparative gene and protein expression during larval development through adulthood. Gene Expr Patterns. Aug; 6(6):637-52.
Laizé V, Viegas CS, Price PA, Cancela ML. (2006) Identification of an osteocalcin isoform in fish with a large acidic prodomain. J Biol Chem. Jun 2; 281(22):15037-15043.
Ortiz-Delgado JB, Simes DS, Viegas CS, Schaff BJ, Sarasquete C, and Cancela ML. (2005) Cloning of matrix Gla protein in a marine cartilaginous fish, Prionace glauca: identification of sites of mRNA and protein accumulation at single cell resolution. Histochem Cell Biol. Dec; 13:1-13.
Pinto JP, Conceição NM, Viegas CS, Leite R, Hurst LD, Kelsh RN, Cancela ML. (2005) Identification of a new pebp2αA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro- J Bone Min Res. Aug; 20(8):1440-53.
Laize V , Martel P, Viegas CS, Price PA, Cancela ML. (2005) Evolution of matrix and bone gamma -carboxyglutamic acid proteins in vertebrates. J Biol Chem. July; 280(29):26659-68.
Simes DC, Williamson MK, Viegas CS, Price PA, Cancela ML. (2003) Purification of Matrix Gla Protein (MGP) from a marine teleost fish, Argyrossomus regius: calcified cartilage and not bone as the primary site of MGP accumalation in fish. J Bone Miner Res. Feb;18(2):244-59.
Viegas CS, Pinto JP, Conceição N, Simes DC, Cancela ML. (2002) Cloning and characterization of the cDNA and gene encoding Xenopus laevis osteocalcin. Gene, 289, 97-107.
Cancela ML, Ohresser MC, Reia JP, Viegas CS, Williamson MK, Price, PA. (2001) Matrix Gla Protein in Xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations. J Bone Miner Res. Sep; 16(9): 1611-21.
PUBLICATIONS IN CONFERENCE PROCEEDINGS
Ana Paula Silva, Carla Viegas, Dina Simes, Filipa Mendes, Nelson Tavares, Fatima Rato, Nelio Santos, Pedro Neves. (2018) Gla-Rich Protein as a novel marker for calcifications in diabetic patients with CKD. Nephrology Dialysis Transplantation. 33 (Suppl_1): i493, https://doi.org/10.1093/ndt/gfy104.SP430.
Viegas CSB, Santos L, Macedo AL, Morais R, Silva AP, Neves PL, Matos AP, Vermeer C, Simes DC. (2017) Circulating calciprotein particles and extracellular vesicles as novel players in chronic kidney disease vascular calcification: a role for Gla-rich protein. Nephrology Dialysis Transplantation. 32(Suppl_3):iii67–iii68, https://doi.org/10.1093/ndt/gfx123.MO055.
Simes DC, Cavaco S, Viegas CSB, Costa R, Rafael MS, Laires R, Marques V, Coelho AV, De los Reyes C, Zubia E (2015) “New Targets for therapeutics in osteoarthritis: Marine compounds and Gla rich proteins”. Arch Med Biomed Res, 2(1-3):1-109. http://www.iambr.info/AMBR.
Macedo AL, Viegas CSB, Matos APA, Simes DC (2016) “Extracellular Vesicles and Circulating Fetuin-Mineral Complexes in Vascular Calcification; Studies by EM Techniques”. Microscopy and Microanalysis 22(S4):16-17. DOI: 10.1017/S1431927616000271
Viegas CSB, Rafael MS, Enriquez JL, Teixeira A, Vitorino R, Costa RM, Santos LR, Neves J, Macedo AL, Vermeer C, Simes DC (2015) “Gla-rich protein (GRP) is a new player in mineralization-competence of extracellular vesicles involved in vascular calcification”. J Vasc Res 52(Suppl 1):1-88.
Marta S. Rafael, Sofia Cavaco, Carla S.B. Viegas, Sofia Santos, Acácio Ramos, Inês M. Luís, Rúben Costa, Brecht A. G. Willems, Marjolein Herfs, Elke Theuwissen, Cees Vermeer, Dina C. Simes (2014) “Involvement of Gla rich protein (GRP) with pathological calcification during osteoarthritis. Insights into its ?-carboxylation status.” Bone 3 PP373. DOI:10.1530/boneabs.3.PP373.
Cavaco S, Viegas CSB, Rafael MS, Ramos A, Morera JL, Teixeira A, Smit E, Herfs M, Vermeer C, Simes DC. (2012) “Gla rich protein (GRP) is associated to osteoarthritis being highly accumulated in the joint tissues and synovial fluid”. Osteoarthritis and Cartilage 20(Suppl 1):S90-S91.
Cavaco SI, Viegas CBS, Cordeiro OD, Vareilles MC, Silva TS, Palma PF, Simes DC (2011) “Identification and characterization of GRP in articular cartilage and synovial fluid using a proteomic-based approach”. Bone 48(Suppl 2): S265.
Simes DC, Viegas CS, Williamson MK, Price PA, Cancela ML. "Gla Rich Protein (GRP), A New Marker For Ectopic Calcifications" (2009) J Bone Miner Res 24 (Suppl 1). Available at http://www.asbmr.org/Meetings/AnnualMeeting/AbstractDetail.aspx?aid=2f78...
Viegas CS, Simes DC, Williamson MK, Laize V, Price PA, Cancela ML. (2008) “Identification of an hybrid gene in the Adriatic sturgeon (Acipenser naccarii), an ancient bony fish with a cartilaginous endoskeleton”. Calcified Tissue International 82: S121-S122.
Simes DC, Viegas CS, Williamson MK, Price PA, Cancela ML. (2008) “Purification of Matrix Gla Protein and Osteocalcin from the Adriatic sturgeon (Acipencer naccarii), an ancient bony fish with a cartilaginous endoskeleton”. Bone 42(Suppl 1) S65.
Gavaia P, Viegas CA, Pinto JP, Sarasquete C, Cancela ML. (2001) “Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry”. Bone, 28 (5) S103.
Pearson GA, Viegas CS, Serrão EA, Cancela ML. (2001) “Plastid Genes and Gene Expression in the Interdial Alga Fucus Vesiculosus L. (Heterokontophyta, Phaeophyceae)”. Eur. J. Biochem. 268 (Suppl.1), p.216.
ORAL COMMUNICATIONS:
Ana Paula Silva, Carla Viegas, Dina Simes, Pedro Leão Neves. (2019) Gla-Rich Protein: um novo marcador cardioprotector numa população de diabéticos com doença renal crónica. Encontro Renal 2019, XXXIII Congresso SPN, March 28-30, Vilamoura, Portugal.
Carla Viegas, Evelina Edelweiss, Justine Schneider, Christine Schaeffer-Reiss, Arnaud Poterszman, Marta Rafael, Nuna Araújo, Anjos Macedo, António Alves de Matos, Dina Simes. (2018) Use of an innovative system and nanotechnology-based strategy for therapeutic applications of Gla-rich protein (GRP). 3rd International Congress of CiiEM- Research and Innovation in Human & Health Sciences, June 20th-22th, Monte da Caparica, Portugal.
Dina Simes, Carla Viegas, Ana Silva, Filipa Brito, Pedro Leão Neves. Gla-rich Protein (GRP): A new player in the burden of vascular Calcification – clinical implications (2018) Núcleo de Biologia Vascular da SPACV October 13, Ílhavo, Portugal.
Nuna Araújo, Carla Viegas, Inês Perrolas, Rúben Costa, Joana Magalhães, Francisco Blanco, Acácio Ramos, Maria Miguel, Cees Vermeer, Eva Zubía, Dina Simes. (2018) Amentadione is a new modulating agent for osteoarthritis in an ex-vivo co-culture preclinical assay. 3rd International Congress of CiiEM- Research and Innovation in Human & Health Sciences, June 20-22, Monte da Caparica, Portugal.
Ana Silva, Filipa Brito, Carla Viegas, Dina Simes, Fátima Rato, Nélio Santos, Pedro Leão Neves. (2018) Gla-Rich Protein como um novo marcador de calcificações vasculares numa população de diabéticos com doença renal crónica. Encontro Renal 2018, XXXII Congresso SPN, March 22-24, Vilamoura, Portugal.
Viegas CSB, Santos L, Macedo AL, Morais R, Silva AP, Neves PL, Matos AP, Vermeer C, Simes DC. (2017) Circulating calciprotein particles and extracellular vesicles as novel players in chronic kidney disease vascular calcification: a role for Gla-rich protein. ERA-EDTA 54th Annual Congress, June 3-6, Madrid, Spain.
Simes DC, Viegas CSB. (2017) Gla-rich Protein (GRP) is a New Therapeutic Target for Chronic Kidney Disease. PCS 4th World Congress of Cardiothoracic-renal Diseases “Bridging Basic Research and Clinical Practice”, November 4-5, Lisbon, Portugal.
Viegas CS, Simes DC. (2017) Unveiling the dual role of Gla-rich protein (GRP) in the crosstalk between inflammation and calcification in pathological processes. CCMAR Seminars, 28th June, University of Algarve, Faro, Portugal.
Simes DC, Viegas CS. (2017) Gla-rich protein, a vitamin K-dependent protein involved in inflammation and calcification-related diseases. Translational Research and Innovation in Human and Health Sciences, 2nd International Congress of CiiEM, 12th June, Lisbon, Portugal.
Simes DC, Viegas CS, Araújo N, Cavaco S, Costa R, Perrolas I, Santos L, Ramos A, Zubia E, (2016) Marine bioactive compounds: testing novel drugs for old desiases IX Xornada Cientifica Terapia Celular e medicina regenerativa, CICA, Campus de Elviña, A Coruña, Spain.
Viegas CS, Macedo AL, Morais R, Santos L, Matos AP, Simes DC (2016) “Dysregulated fetuin-mineral complexes are linked to vascular calcification in chronic kidney disease: the role of Gla-rich protein”. Ultrapath XVII, Society for Ultrastructural Pathology, 11-15 July, Lisbon, Portugal.
DC Simes, S Cavaco, CSB Viegas, R Costa, MS Rafael, R Laires, V Marques, AV Coelho, C De los Reyes, E Zubía (2015) "New targets for therapeutics in osteoarthritis: Marine compounds and Gla rich proteins". 9th SFRR-Africa/4th IAMBR conference. Translational Science and Drug Discovery: Impact on Health, Wellness, Environment and Economics, 27-29 July Mauritius.
Viegas CSB, Rafael MS, Enriquez JL, Teixeira A, Vitorino R, Costa RM, Santos LR, Neves J, Macedo AL, Vermeer C, Simes DC (2015) “Gla-rich protein (GRP) is a new player in mineralization-competence of extracellular vesicles involved in vascular calcification”. Joint Meeting of the European Society for Microcirculation (ESM) and European Vascular Biology Organization (EVBO), June 3-6, Pisa, Italy.
Ramos A, Cavaco S, Sacramento T, Gomes D, Viegas C, Simes DS. "Papel de uma nova proteina no processo degenerativo da cartilagem articular: resultados preliminares". XXXI Congresso Nacional de Ortopedia e Traumatologia 19-21 de Outubro, Centro de Congressos do Estoril, Estoril, Portugal.
Cancela ML, Viegas CS, Laizé V, Simes DC. (2009) “Fish as model organisms to uncover new Gla proteins of interest for human biology”. Workshop - Interdisciplinary Approaches in Fish Skeletal Biology, 27-29 April, Tavira, Portugal.
Viegas CS, Simes D, Williamson MK, Laize V, Price PA, Cancela ML. (2008) Identification of an MGP/OC hybrid gene in the Adriatic sturgeon (Acipenser naccarii), representing an ancient form of osteocalcin”. Seminários Mar e Ambiente, University of Algarve, May 8.
Viegas CS, Simes D, Cancela ML. (2007) “Identification of an MGP/OC hybrid gene in Adriatic sturgeon Acipenser naccarii”. Seminários Mar e Ambiente, May 3-4.
Conceição NM, Viegas CS, Fidalgo J, Laizé V and Cancela ML. (2004) “Identification of two distinct promoter regions in fish MGP genes. Implications for regulation of MGP gene expression”. XIV Congresso Nacional de Bioquímica, Vilamoura, Portugal, 2-4 Dezembro.
Laizé L, Martel P, Viegas CS, Cancela ML. (2004) “Evolutionary relationship between bone and matrix Gla proteins”. XIV Congresso Nacional de Bioquímica, Vilamoura 2-4 Dezembro.
Cavaco SI, Simes DC, Viegas CS, Pombinho AJ, Leite RB, Cancela ML. (2004) “Effect of nacre extracts from the oyster Crassostrea gigas in the mineralization of bone-derived cells from Sparus aurata”. XIV Congresso Nacional de Bioquímica, Vilamoura, Portugal.
Viegas CS (2012). “Applications of Recombinant Proteins- Biomedical Perspective and Beyond”. I Ciclo de Palestras, Núcleo de Estudantes de Engenharia Biológica e Biotecnologia, November 26.
Viegas CS (2012).“Applications of recombinant proteins”. Workshop on Strategies for recombinant protein production, July11-12, University of Algarve.
Simes DC, Viegas CS, Cavaco S, Neves PL, Ferreira A, João A, Williamson MK, Price PA, Cancela ML. (2010) “Gla Rich Protein (GRP), A New Biomarker For Ectopic Calcifications?”. BIT’s 3rd Annual Protein and Peptide Conference, 21-23 March, Beijing, China.
Simes DC, Viegas CS, Cavaco S, Cancela ML, Neves PL, Ferreira A, João A, Williamson MK, Price PA (2010) "Study of Gla Rich Protein (GRP) function in pathological calcification processes and its potential for use as a biomarker". IV SPB Clinical Biochemistry Workshop, 29 January, Faro, Portugal.
Simes DC, Viegas CS, Cancela ML (2009) "Aplicações da análise proteomica 2D na identificação de possíveis biomarcadores para diagnóstico/prognóstico de doenças". Palestra Hospital de Faro 26 October, Faro, Portugal.
Viegas CS, Simes D, Williamson MK, Laize V, Price PA, Cancela ML. (2008) Identification of an MGP/OC hybrid gene in Adriatic sturgeon (Acipenser naccarii), representing an ancient form of Osteocalcin. CCMAR Seminars, November 12, University of Algarve, Faro, Portugal
Cancela ML, Laizé V, Simes DC, Gavaia PJ, Conceição N, Viegas CS, Pombinho AR, Marques SMP, Fidalgo J, Braga D (2005) “Proteínas Gla do osso e cartilagem: importância dos anfíbios e peixes como modelos biológicos para elucidação da sua função e evolução”. 1º Encontro “O Mar e as Ciências da Saúde”, Caparica 21 de Abril
Gavaia PJ, Viegas CS, Pinto JP, Sarasquete MC, Cancela ML. (2001) “Detection and localization of osteocalcin (BGP or Bone Gla protein) in Teleosts by in situ hybridization and immunohistochemistry.” Simpósio Satélite da Sociedade Espanhola de Investigação Óssea e Metabolismo Mineral. 1st Joint Meeting of the International Bone and Mineral Society and the European Calcified Tissue Society. Madrid, Spain 5-10 Junho
POSTER COMMUNICATIONS:
Ana Paula Silva, Carla Viegas, Dina Simes, Filipa Mendes, Nelson Tavares, Fatima Rato, Nelio Santos, Pedro Neves. (2018) Gla-Rich Protein as a novel marker for calcifications in diabetic patients with CKD. 55th ERA-EDTA Congress, May 24-27, Copenhagen, Denmark.
Araújo N, Viegas CSB, Pontes JF, Magalhães J, Blanco FJ, Macedo AL, Grenha A, Simes DC. (2017) Nano-encapsulation of Gla-rich protein (GRP) and potential added value as a therapeutic agent for osteoarthritis. 21st International Symposium on Microencapsulation, September 27-29, University of Algarve, Faro, Portugal, PT67.
Araújo N, Perrolas I, Costa R, Viegas CS, Ramos A, Zubia E, Simes DC. (2016) Exploring meroterpenoids from brown algae as novel therapeutic drugs for osteoarthritis, in the XV International Symposium on Marine Natural Products – MaNaPro, 29th August to 2nd September, Cumbuco, Ceará, Brazil, Poster talk and PT12.
Morais R, Viegas CS, Simes DC, Matos AP, Macedo AL (2016) “Application of TEM Techniques for the Study of Vascular Calcification: Monitoring Extracellular Vesicles and NanoGold Immunodection of Fetuin-A, GRP and CD9”. Ultrapath XVII, Society for Ultrastructural Pathology, 11-15 July, Lisbon, Portugal.
Morais R, Macedo AL, Viegas CSB, Matos APA, Simes DC (2015) “Electron Microscopy Contribution to the Study of Vascular Calcification Process: Monitoring Extracellular Vesicles Biogenesis and Mineralization”. First International Congress of CiiEM. From Basic Sciences to Clinical Research, 27-28 November, Caparica-Lisboa, Portugal.
Macedo AL, Viegas CSB, Matos APA, Simes DC (2015) “Extracellular Vesicles and Circulating Fetuin-Mineral Complexes in Vascular Calcification; Studies by EM Techniques”. 4th Joint Congress of the Portuguese and Spanish Microscopy Societies: Microscopy at the Frontiers of Science (MFS), 9-11 September, Porto, Portugal.
Santos LR, Rafael MS, Viegas CS & Simes DC (2014) "Studies towards unveiling potential interaction partners of Gla-rich protein (GRP), a potential regulator of pathological calcification related diseases." in XVIII Congress of the Portuguese Biochemical Society, 17-20 December, Coimbra, Portugal.
Costa RM, Viegas CS, Rafael MS, Marques G, Silva Z, Videira P and Simes DC (2014) “Study of mineralization-related vitamin K-dependent proteins associated with leukocytes in mineralization and inflammatory processes.” in XVIII Congress of the Portuguese Biochemical Society, 17-20 December, Coimbra, Portugal.
Marta S. Rafael, Sofia Cavaco, Carla S.B. Viegas, Sofia Santos, Acácio Ramos, Inês M. Luís, Rúben Costa, Brecht A. G. Willems, Marjolein Herfs, Elke Theuwissen, Cees Vermeer, Dina C. Simes (2014) “Involvement of Gla rich protein (GRP) with pathological calcification during osteoarthritis. Insights into its ?-carboxylation status.” European Calcified Tissue Society, 17-20 May. Prague, Czech Republic.
Rafael MS, Viegas CSB, Ferrer-Miralles N, Vermeer C, Simes DC. (2012) Production of functional recombinant Gla-rich protein (GRP) for clinical diagnosis, Practical workshop on Production, purification and characterization of macromolecular complexes, 26 Nov. – 1 Dec. IGBMC, Strasbourg, France.
Cavaco S, Viegas CSB, Rafael MS, Ramos A, Morera JL, Teixeira A, Smit E, Herfs M, Vermeer C, Simes DC. (2012) “Gla rich protein (GRP) is associated to osteoarthritis being highly accumulated in the joint tissues and synovial fluid”. OARSI 2012 World Congress, Barcelona, Spain.
Viegas C, Cavaco S, Ramos A, Silva J, Morera J, Teixeira A, Smit E, Herfs M, Vermeer C, Simes D (2011) “Insights into the relation between Gla rich protein (GRP) and Osteoarthritis”. FASEB Summer Research Conferences on Molecular, Structural & Clinical Aspects of Vitamin K & Vitamin K Dependent Proteins, June 26 - July 1, Carefree, Arizona.
Viegas C, Morera J, Teixeira A, João A, Ferreira A, Neves P, Smit E, Herfs M, Vermeer C, Simes DS (2011) “GRP is a new VKD protein associated with ectopic calcifications and potential application in clinical diagnostics”. FASEB Summer research conferences- Molecular, Structural and clinical aspects of Vitamin K and Vitamin K dependent Proteins, 26 June-1 July, Carefree, Arizona, USA.
Smit E, Herfs M, Viegas C, Simes DS, Vermeer C (2011) "GRP: a new Vitamin K dependent protein, immunolocalization in various human tissues". FASEB Summer research conferences- Molecular, Structural and clinical aspects of Vitamin K and Vitamin K dependent Proteins, 26 June-1 July, Carefree, Arizona, USA.
Cavaco SI, Viegas CBS, Cordeiro OD, Vareilles MC, Silva TS, Palma PF, Simes DC (2011) “Identification and characterization of GRP in articular cartilage and synovial fluid using a proteomic-based approach”. 3rd Joint Meeting of the European Calcified Tissue Society & the International Bone and Mineral Society, 7-11 May, Athenas, Greece.
Cavaco SF, Viegas CSB, Simes DC. (2010) "2-DE Proteomics of human articular cartilage for the identification of osteoarthritis biomarkers - procedures optimization", "4th EUPA meeting and 6th ProCura meeting", 23-27 Outubro, Estoril, Portugal.
Viegas CS, Cavaco S, Neves PL, Ferreira A, João A, Williamson MK, Price PA, Cancela ML, Simes DC. (2010) “GRP is a New Vitamin K Dependent Protein and a Possible Ectopic Calcification Biomarker with Diagnostic Implications”. BIT’s 3rd Annual Protein and Peptide Conference, 21-23 March, Beijing, China.
Fazenda C, Viegas CS, Conceição N, Simes DC, Cancela ML. (2009) “Development of molecular and cellular tools to study the function of skeletal Gla-containg proteins in sturgeon”. Workshop - Interdisciplinary Approaches in Fish Skeletal Biology, 27-29 April, Tavira, Portugal.
Viegas CS, Simes DC, Williamson MK, Laize V, Price PA, Cancela ML. (2008) “Identification of an hybrid gene in the Adriatic sturgeon (Acipenser naccarii), an ancient bony fish with a cartilaginous endoskeleton” 35th ECTS Meeting, 24-28 May, Barcelona, Spain.
Simes DC, Viegas CS, Williamson MK, Price PA, Cancela ML. (2008) “Purification of Matrix Gla Protein and Osteocalcin from the Adriatic sturgeon (Acipencer naccarii), an ancient bony fish with a cartilaginous endoskeleton”. The International Bone and Mineral Society, March 9-14, Davos, Switzerland.
Laizé V, Viegas CS, Simes DC, Cancela ML. (2008) “Evolutionary relationship of bone-associated Gla proteins” 2nd meeting of the European Society for Evolutionary Development Biology, July 29 - August 1, Ghent, Belgium.
Simes DC, Viegas CS, Williamson MK, Price PA, Cancela ML. (2008) “Purification of Matrix Gla Protein and Osteocalcin from the Adriatic sturgeon (Acipencer naccarii), an ancient bony fish with a cartilaginous endoskeleton”. The International Bone and Mineral Society, 9-14 March, Davos, Switzerland. In: Bone S106.
Viegas CS, Gavaia P, Simes D, Cancela ML. (2006). “Molecular cloning and regulation of the genes encoding Matrix and Bone Gla proteins from Sturgeon. Studies towards understanding the evolution of their molecular mechanism of action”. Seminários Mar e Ambiente, May 19-20.
Laize V, Martel P, Viegas CS, Cancela ML. (2005). “Conserved Features of Matrix and Bone Gamma-Carboxyglutamic Acid Proteins in Vertebrates - An Evolutionary Point of View” 2nd Joint Meeting f the European Calcified Tissue Society (ECTS) and International Bone and Mineral Society (IBMS). Geneva, Switzerland, June 25-29.
Mariani V, Viegas CS, Gavaia PJ, Cancela ML & Giuffra E. (2005) “Comparative Analysis of Gene Expression Profiles Among Distantly Related Fish Species. Insight into Skeletal and Immunological Systems”. XIII Conference on Plant & Animal Genomes, San Diego, California, USA 15-19 Janeiro.
Gavaia P, Laizé V, Mariani V, Viegas CS, Giuffra E, Cancela ML. (2004) “Comparative analysis of gene expression profiles among distantly related fish species. Insight into skeletal and immunological systems”.Marine Genomics Europe Kick-off Meeting, Rennes, França 22-24 Junho.
Brito A, Ronnestad I, Gavaia PJ, Viegas CS, Cancela ML. (2004) “Expression of the oligopeptide transporter PepT1 (solute carrier family 15, member1) in fed and starved larvae of zebra fish (Danio rerio)”. XIV Congresso Nacional de Bioquímica, Vilamoura 2-4 Dezembro.
Ronnestad I, Gavaia PJ, Viegas CS, Cancela ML. (2004) “Cloning and ontogenic expression of the oligopeptide transporter PepT1 (solute carrier family 15, member1) in fed and starved larvae of atlantic cod, Gadus morhua L.” XIV Congresso Nacional de Bioquímica, Vilamoura 2-4 Dezembro.
Viegas CS, Gavaia PJ, Ortiz-Delgado JB, Sarasquete C, Cancela ML. (2004) “Gla proteins as markers for studies of skeletal development and malformations in new aquaculture species (Pagrus auriga, Diplodus sargo and Scophtalmus maximus)”. XIV Congresso Nacional de Bioquímica, Vilamoura 2-4 Dezembro.
Simes DC, Ortiz-Delgado JB, Viegas CS, Schaff B, Cancela ML. (2004) “Cloning of matrix Gla protein in a marine cartilaginous fish, Prionace glauca: identification of sites of mRNA and protein accumulation at single cell resolution”. XIV Congresso Nacional de Bioquímica, Vilamoura 2-4 Dezembro
Simões B, Conceição N, Viegas CS, Pinto J, Cancela ML. (2004) “Molecular cloning of the zebra fish (Danio rerio) Collagen Xa1 cDNA and gene. Identification of promoter regulatory elements”. XIV Congresso Nacional de Bioquímica, Vilamoura 2-4 Dezembro.
Pinto JP, Conceição NM, Viegas CS, Leite RB, Kelsh RN and Cancela ML. “Identification of a new pebp2aA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro”. XIV Congresso Nacional de Bioquímica, Vilamoura, Portugal, 2-4 Dezembro, 2004.
Pinto JP, Viegas CS, Simes D, Rodrigues P, Macedo A, Goodfellow B, Cancela ML. (2000) “Identification of invariant amino acid residues in BGP from fish to mammals suggests a conserved function throughout vertebrate evolution.” 20th Conference of European Comparative Endocrinologists, Faro, Portugal 5-9 Setembro.
Pearson GA, Viegas CS, Serrão EA, Cancela ML. (2001) “Plastid genes and gene expression in the intertidal alga Fucus vesiculosus L. (heterokontophyta, phaeophyceae)”. 27th FEBS Meeting, Lisboa, Portugal 30 Junho-5 Julho.
Pearson GA, Viegas CS, Serrão EA, Cancela ML. (2001) “Plastid genes and gene expression in the intertidal alga Fucus vesiculosus L.(heterokontophyta, phaeophyceae).” (vencedor do “George F. Papenfuss award” para a melhor apresentação em painel em “Physiological and Ecological interactions”). 7th International Phycological Congress, Thessaloniki, Grécia 18-25 Agosto.
Viegas CS, Lopes EP, Pinto JP, Cancela ML. (2000) “Bone Gla protein in Xenopus laevis: molecular cloning and tissue distribution”. XII Congresso Nacional de Bioquímica, Póvoa do Varzim 28-30 Setembro.
PATENTS:
Simes DC, Viegas, CS, and Cancela ML. Provisional U.S. Patent Application of University of Algarve - Serial number 61136.315; filled on August 27, 2008. PCT Patent application: PCT/PT2009000046, August 27, 2009.
PATENTSCOPE publication number WO/2010/024704 - A gammacarboxyglutamate-rich protein, methods and assays for its detection, purification and quantification and uses thereof. Publication date: 04.03.2010
Patent PCT/PT2009/000046 Title: "A GAMMACARBOXYGLUTAMATE-RICH PROTEIN, METHODS AND ASSAYS FOR ITS DETECTION, PURIFICATION AND QUANTIFICATION AND USES THEREOF" Entry in the National phase of PCT/PT2009/000046 in EUA; Request number: 13/061,317; Entry date: 28 February 2011; Priority date: 27 August 2008 (US 61/136,315).
CT/PT2009/000046 National Phase entries:
- European Patent Office (EPO) Entry date 25.02.2011; National number 2009737193; National status Published: 01.06.2011.
- United States of America Office Entry date 19.05.2011; National number 13061317, National status Published: 01.09.2011.
