NEW Date: 11th-12th July

Introduction: Proteins are the engines of biological systems and nearly all pharmaceuticals act on proteins. Also, proteins themselves are increasingly used therapeutically. As biology enters the post-genomic era, researchers have begun to embrace the exciting opportunity of investigating proteins in high throughput experiments. The study of proteins includes a vast array of techniques ranging from enzyme catalysis assays to interaction and structural studies. Many of these methods depend on purified protein availability sometimes difficult to isolate from native sources. Alternative Biotechnology offers ways to overcome this problem and allow the production and purification of high quantities of functional proteins. The high diversity of expression systems and improved purification techniques available nowadays, assure the production of virtually any functional protein, which become a powerful tool both in fundamental research and in applied therapeutics.

Course description: The course is intended for students and researchers (Bachelor/MSc /PhD) who wish to learn about the process of production of recombinant proteins and are willing to gather more information on currently available expression systems and protein purification techniques. The course includes lectures on advanced and cutting-edge techniques used for heterologous protein production and purification. The aim of the course is to provide an in-depth evaluation of strategies to achieve maximum optimization of recombinant protein production and purification experiments.
Participants: PhD/ Master/Graduate students and researchers interested in the field of heterologous protein production and purification.

The course will be given in English.

Registration: 13 euros payment fee. For information and registration click here.

Location: Amph. C (Complexo pedagógico - building 3 - Gambelas/UAlg)

Detailed program:

Day 1
9 am - 10 am - Registration

10 am -10:15 am - Welcoming speech

10:15 - 11:30 am - Global approach to recombinant proteins production and applications- Neus Ferrer (Autonomous Univ of Barcelona)

11:30-12:45 am - Expression systems: Escherichia coli, insect cells, and mammalian cells- Advantages and disadvantages - Neus Ferrer

14:30 pm - 16:00 pm - Overview of a study design - Neus Ferrer

Day 2
9 - 10 am - Strategies to express proteins in plants - Gustavo Nolasco (FCT-UALG)
10 -11:30 am - Basic principles in protein purification - Neus Ferrer (Autonomous Univ. of Barcelona).
Strategy design to optimize protein purification - Neus Ferrer

11:30-12:30 am - Applications of recombinant proteins - Carla Viegas (FCT/CCMAR)

12:30 pm-13:00 pm - Discussion and final remarks

Download Programme

Click to register

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