Spectrophotometry | - CCMAR -

Spectrophotometry

Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through a sample solution. It is one of the most useful methods of quantitative analysis in various fields such as chemistry, physics, biochemistry, material and chemical engineering and clinical applications.

Infrared Microscopy (µ-FTIR) - Thermo Scientific Nicolet iN10MX

The system is a Thermo Scientific instrument, model Nicolet™ iN™10, equipped with DTGS, MCT-A/MCT-B single element and MCT-A Array detectors. Sampling can be performed by ATR, reflection and transmission. 

It is a primary tool for compound identification. Provides information on the structure of the main organic and inorganic compounds in each sample, with minimum sample preparation.

Access: Onsite (with training) and Remote

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Multi-Mode Microplate Reader - (Biotek Synergy Neo 2)

 

System with a monochromator for variable bandwidth, absorbance, fluorescence and luminescence filters for fluorescence polarization, time-resolved fluorescence. The equipment allow wavelength selection from 230 to 999 nm in 1-nm increments. Endpoint, area, spectral scanning, and pathlength correction are available.

Two types of fluorescence detection system: filter and monochromator-based. The fluorescence monochromator system provides wavelength selection in 1-nm increments in the range of 250-900 nm (excitation) and 250-900 nm (emission). The luminescence system provides wavelength selection in 1-nm increments in the range of 300 to 700 nm. 

Access: Onsite and Remote

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Multi-Mode Microplate Reader - (Biotek Synergy 4)

The absorbance monochromator provides wavelength selection from 200 to 999 nm in 1-nm increments.
Area and spectral scanning, and pathlength correction read methods are available .

The fluorescence monochromator system provides wavelength selection in 1-nm increments in the range of 250-750 nm (excitation) and 300-800 nm (emission).

The luminescence system provides wavelength selection in 1-nm increments in the range of 300 to 700 nm. 
The Synergy supports the reading of 1-, 6-, 12-, 24-, 48-, 96-, 384-, and 1536-weel microplates, and 96-well half-area plates with 128x86 mm geometry.

Access: Onsite and Remote

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UV-VIS Spectrophotometer (Agilent Cary 4000)

The Cary 4000 is a high-end UV-Vis spectrophotometer with photometric performance in the 175–900 nm range.

The system is ideal for analyzing liquid and solid samples or biotechnology samples with minimal sample preparation.

The equipment has a versatile suite of accessories including temperature control, liquid, and solid sample holders, multicell holders, and diffuse and specular reflectance accessories. 

Access: Remote and onsite
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UV-VIS Spectrophotometer  (Hitachi Model: U2000)

Hitachi Model: U2000

UV-Vis spectrophotometer with wavelength range 190 to 1100nm, spectral bandpass 2nm, and double beam photometric system. This system is ideal for analysing single liquid samples with cuvette vials. 

Access: Remote and onsite

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