Journal Article
| Title | Calcium-transport in quiescent and 1,25-dihydroxycholecalciferol-stimulated human osteosarcoma cells. Role in 24 hydroxylase enhancement. |
| Publication Type | Journal Article |
| Authors | Rebut-Bonneton, C, Ly, SY, M. Cancela, L |
| Year of Publication | 1988 |
| Journal | Horm Metab Res |
| Volume | 20 |
| Issue | 6 |
| Date Published | 1988 Jun |
| Pagination | 339-43 |
| ISSN | 0018-5043 |
| Keywords | Calcitriol, Calcium, Cycloheximide, Cytochrome P-450 Enzyme System, Humans, Nicardipine, Osteosarcoma, Protein Biosynthesis, Proteins, Steroid Hydroxylases, Tumor Cells, Cultured, Vitamin D3 24-Hydroxylase |
| Abstract | The present study evaluates in osteosarcoma cells, the effects of a calcium channel inhibitor nicardipine in 24-hydroxylase activity and 45Ca desaturation curve in presence of 1,25-dihydroxycholecalciferol (1,25(OH)2D3). This sterol induced an increase in 24-OHase activity and 45Ca fluxes. Nicardipine reversed the effect of 1,25(OH)2D3 on 45Ca fluxes but reinforced the enhancement of the 24-OHase activity. The fact that the effects of 1,25(OH)2D3 were reduced by cycloheximide support the hypothesis of a de novo protein synthesis. Our study has allowed us to dissociate the effects of 1,25(OH)2D3 on 24-OHase enhancement from those on Ca2+ transport. |
| DOI | 10.1055/s-2007-1010831 |
| Sapientia | |
| Alternate Journal | Horm. Metab. Res. |
| PubMed ID | 3166448 |
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