Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1μvar): Mortality, viral load and detection of viral transcripts by in situ hybridization. | - CCMAR -

Journal Article

TitleExperimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1μvar): Mortality, viral load and detection of viral transcripts by in situ hybridization.
Publication TypeJournal Article
AuthorsSanmartín, MLópez, Power, DM, de la Herrán, R, Navas, JI, Batista, FM
Year of Publication2016
JournalVirus Res
Volume217
Date Published2016 Jun 2
Pagination55-62
ISSN1872-7492
Abstract

Ostreid herpesvirus 1 (OsHV-1) infections have been reported in several bivalve species. Mortality of Pacific oyster Crassostrea gigas spat has increased considerably in Europe since 2008 linked to the spread of a variant of OsHV-1 called μvar. In the present study we demonstrated that O. edulis juveniles can be infected by OsHV-1μvar when administered as an intramuscular injection. Mortality in the oysters injected with OsHV-1μvar was first detected 4 days after injection and reached 25% mortality at day 10. Moreover, the high viral load observed and the detection of viral transcripts by in situ hybridization in several tissues of dying oysters suggested that OsHV-1μvar was the cause of mortality in the O. edulis juveniles. This is therefore the first study to provide evidence about the pathogenicity of OsHV-1μvar in a species that does not belong to the Crassostrea genus. Additionally, we present a novel method to detect OsHV-1 transcripts in infected individuals' using in situ hybridization.

DOI10.1016/j.virusres.2016.01.023
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/26945849?dopt=Abstract

Alternate JournalVirus Res.
PubMed ID26945849
CCMAR Authors