|Title||Maternal thyroid hormones are essential for neural development in zebrafish.|
|Publication Type||Journal Article|
|Authors||Campinho, MA, Saraiva, JL, Florindo, C, Power, DM|
|Year of Publication||2014|
|Date Published||2014 Jul|
|Keywords||Animals, Brain, Cell Differentiation, Monocarboxylic Acid Transporters, Morpholinos, Neurogenesis, Neurons, Paired Box Transcription Factors, Signal Transduction, Spinal Cord, Thyroid Hormone Receptors alpha, Thyroid Hormone Receptors beta, Thyroid Hormones, Zebrafish, Zebrafish Proteins|
Teleost eggs contain an abundant store of maternal thyroid hormones (THs), and early in zebrafish embryonic development, all the genes necessary for TH signaling are expressed. Nonetheless the function of THs in embryonic development remains elusive. To test the hypothesis that THs are fundamental for zebrafish embryonic development, an monocarboxilic transporter 8 (Mct8) knockdown strategy was deployed to prevent maternal TH uptake. Absence of maternal THs did not affect early specification of the neural epithelia but profoundly modified later dorsal specification of the brain and spinal cord as well as specific neuron differentiation. Maternal THs acted upstream of pax2a, pax7, and pax8 genes but downstream of shha and fgf8a signaling. The lack of inhibitory spinal cord interneurons and increased motoneurons in the mct8 morphants is consistent with their stiff axial body and impaired mobility. The mct8 mutations are associated with X-linked mental retardation in humans, and the cellular and molecular consequences of MCT8 knockdown during embryonic development in zebrafish provides new insight into the potential role of THs in this condition.
|Alternate Journal||Mol. Endocrinol.|