Effects of preslaughter stress levels on the post-mortem sarcoplasmic proteomic profile of gilthead seabream muscle. | - CCMAR -

Journal Article

TitleEffects of preslaughter stress levels on the post-mortem sarcoplasmic proteomic profile of gilthead seabream muscle.
Publication TypeJournal Article
AuthorsSilva, TS, Cordeiro, OD, Matos, E, Wulff, T, Dias, JP, Jessen, F, Rodrigues, P
Year of Publication2012
JournalJ Agric Food Chem
Volume60
Issue37
Date Published2012 Sep 19
Pagination9443-53
ISSN1520-5118
KeywordsAnimals, Aquaculture, Electrophoresis, Gel, Two-Dimensional, Food Quality, Muscle Proteins, Muscles, Oxidative Stress, Postmortem Changes, Proteolysis, Proteomics, Sarcoplasmic Reticulum, Sea Bream, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Stress, Psychological
Abstract

Fish welfare is an important concern in aquaculture, not only due to the ethical implications but also for productivity and quality-related reasons. The purpose of this study was to track soluble proteome expression in post-mortem gilthead seabream muscle and to observe how preslaughter stress affects these post-mortem processes. For the experiment, two groups of gilthead seabream (n = 5) were subjected to distinct levels of preslaughter stress, with three muscle samples being taken from each fish. Proteins were extracted from the muscle samples, fractionated, and separated by 2DE. Protein identification was performed by MALDI-TOF-TOF MS. Analysis of the results indicates changes on several cellular pathways, with some of these changes being attributable to oxidative and proteolytic activity on sarcoplasmic proteins, together with leaking of myofibrillar proteins. These processes appear to have been hastened by preslaughter stress, confirming that it induces clear post-mortem changes in the muscle proteome of gilthead seabream.

DOI10.1021/jf301766e
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/22906076?dopt=Abstract

Alternate JournalJ. Agric. Food Chem.
PubMed ID22906076
CCMAR Authors