|Title||Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.|
|Publication Type||Journal Article|
|Authors||Pereira, H, Barreira, L, Mozes, A, Florindo, C, Polo, C, Duarte, CV, Custódio, L, Varela, J|
|Year of Publication||2011|
|Date Published||2011 Dec 22|
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.
|Alternate Journal||Biotechnol Biofuels|
|PubMed Central ID||PMC3287250|