|Title||Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range.|
|Publication Type||Journal Article|
|Authors||Massa, SI, Pearson, GA, Aires, T, Kube, M, Olsen, JL, Reinhardt, R, Serrão, EA, Arnaud-Haond, S|
|Year of Publication||2011|
|Date Published||2011 Sep|
|Keywords||Biological Evolution, Climate Change, Demography, Expressed Sequence Tags, Flavoproteins, Gene Expression Regulation, Plant, Glutamate-Ammonia Ligase, Glyceraldehyde-3-Phosphate Dehydrogenases, Hot Temperature, Microsatellite Repeats, Oxidative Stress, Oxidoreductases Acting on CH-CH Group Donors, Peptidylprolyl Isomerase, Photosynthesis, Plant Proteins, Transcriptome, Zosteraceae|
Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera noltii during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30min, 2h, 4h and 24h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as 'molecular chaperones' and were over-expressed following heat-shock, while 12 (23.5%) were 'photosynthesis TUGs' generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30min AHS library, followed by a slow decrease during further recovery. In contrast, 'photosynthesis TUGs' were under-expressed 30min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified.
|Alternate Journal||Mar Genomics|