Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.). | - CCMAR -

Journal Article

TitleMelatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.).
Publication TypeJournal Article
AuthorsAbbink, W, Kulczykowska, E, Kalamarz, H, Guerreiro, PM, Flik, G
Year of Publication2008
JournalGen Comp Endocrinol
Volume155
Issue1
Date Published2008 Jan 1
Pagination94-100
ISSN0016-6480
KeywordsAnimals, Brain, Brain Chemistry, Calcium, Diet, Hypocalcemia, Melatonin, Sea Bream
Abstract

Brain or blood plasma melatonin was analysed as a measure for pineal melatonin production in sea bream. Access to calcium was limited by diluting the seawater to 2.5 per thousand and removing calcium from the diet or by prolonged feeding of vitamin D-deficient diet. Interactions/relations between melatonin and calcium balance and the hypercalcemic endocrines PTHrP and calcitriol were assessed. Restricting calcium availability in both water and diet had no effect on plasma melatonin, but when calcium was low in the water or absent from food, increased and decreased plasma melatonin was observed, respectively. Fish on a vitamin D-deficient diet (D- fish) showed decreased plasma calcitriol levels and remained normocalcemic. Decreased brain melatonin was found at all sampling times (10-22 weeks) in the D- fish compared to the controls. A positive correlation between plasma Ca2+ and plasma melatonin was found (R(2)=0.19; N=41; P <0.01) and brain melatonin was negatively correlated with plasma PTHrP (R(2)=0.78; N=4; P <0.05). The positive correlation between plasma levels of melatonin and Ca2+ provides evidence that melatonin synthesis is influenced by plasma Ca2+. The decreased melatonin production in the D- fish points to direct or indirect involvement of calcitriol in melatonin synthesis by the pineal organ in teleosts. The hypercalcemic factors PTHrP and calcitriol appeared to be negatively correlated with melatonin and this substantiates an involvement of melatonin in modulating the endocrine response to cope with hypocalcemia. It further points to the importance of Ca2+ in melatonin physiology.

DOI10.1016/j.ygcen.2007.03.002
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/17434515?dopt=Abstract

Alternate JournalGen. Comp. Endocrinol.
PubMed ID17434515