A cDNA for European sea bass (Dicentrachus labrax) 11beta-hydroxylase: gene expression during the thermosensitive period and gonadogenesis. | - CCMAR -

Journal Article

TitleA cDNA for European sea bass (Dicentrachus labrax) 11beta-hydroxylase: gene expression during the thermosensitive period and gonadogenesis.
Publication TypeJournal Article
AuthorsSocorro, S, Martins, RS, Deloffre, L, Mylonas, CC, Canario, AVM
Year of Publication2007
JournalGen Comp Endocrinol
Volume150
Issue1
Date Published2007 Jan 1
Pagination164-73
ISSN0016-6480
KeywordsAnimals, Aromatase, Base Sequence, Bass, DNA, Complementary, Female, Gene Expression Regulation, Developmental, Male, Molecular Sequence Data, Organogenesis, RNA, Messenger, Sex Differentiation, Steroid 11-beta-Hydroxylase, Temperature, Testis
Abstract

Steroid P450 11beta-hydroxylase, encoded by the CYP11B gene, is a key mitochondrial enzyme for the production of 11-oxygenated androgens, which have been shown to be potent masculinising steroids in several fish species. In this study we have isolated a CYP11B cDNA of 1903 base pairs from the testis of European sea bass (Dicentrarchus labrax) encoding a predicted protein of 552 amino acids. The amino acid identities to other vertebrate 11beta-hydroxylase proteins ranged from 66% to 72% to other fish; 45% to amphibian and 35-39% to mammalian. Southern blot indicated that a single CYP11B gene is present. Northern blot analysis detected two transcripts in testis and head kidney, one of the same size of the isolated clone and the other of 3.9 kb. Reverse transcriptase-polymerase chain reaction showed abundant mRNA expression only in testis and head kidney, being residual in a range of other tissues. Expression of CYP11B and CYP19A (which encodes for ovarian aromatase) was detected from at least 4 days post-hatching and did not appear to be affected by rearing temperature (15 and 20 degrees C) during the first 60 days, a period in which high temperatures promote masculinisation in European sea bass. Throughout, gonadogenesis (60-300 dph), a highly dimorphic pattern of CYP11B expression was consistent with a role of this gene in testicular development.

DOI10.1016/j.ygcen.2006.07.018
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/16962121?dopt=Abstract

Alternate JournalGen. Comp. Endocrinol.
PubMed ID16962121
CCMAR Authors