|Title||CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein.|
|Publication Type||Journal Article|
|Authors||Bevelander, GS, Pinto, ESLC, Canario, AVM, Spanings, T, Flik, G|
|Year of Publication||2008|
|Date Published||2008 Mar|
|Keywords||Amino Acid Sequence, Animals, Base Sequence, Calcitriol, Cholestanetriol 26-Monooxygenase, Cloning, Molecular, DNA, Complementary, Gene Expression Regulation, Enzymologic, Male, Molecular Sequence Data, Parathyroid Hormone-Related Protein, Phylogeny, RNA, Messenger, Sea Bream, Sequence Homology, Amino Acid, Vitamin D, Vitamins|
Little is known about vitamin D metabolism in fishes. Several reports have shown hydroxylase activities in various organs to produce vitamin D metabolites, but the enzymes involved have not been isolated or characterized. We isolated and characterized a renal mitochondrial hydroxylase, CYP27A1, that governs vitamin D metabolism in gilthead sea bream, Sparus auratus. The enzyme is highly expressed in kidney and to a far lesser extent in liver. When treated with 25-hydroxy vitamin D or calcitriol, the kidney responded differentially and time dependently with CYP27A1 mRNA expression levels. This response substantiates a role for CYP27A1 in fish vitamin D metabolism. This notion is strengthened by upregulation of CYP27A1 in sea bream treated with parathyroid hormone-related protein (PTHrP), and suggests an original role for PTHrP in calcitriol-regulated processes n fish similar to the role of PTH in mammalian vitamin D-dependent processes.
|Alternate Journal||J. Endocrinol.|