Brain aromatase from pejerrey fish (Odontesthes bonariensis): cDNA cloning, tissue expression, and immunohistochemical localization. | - CCMAR -

Journal Article

TitleBrain aromatase from pejerrey fish (Odontesthes bonariensis): cDNA cloning, tissue expression, and immunohistochemical localization.
Publication TypeJournal Article
AuthorsStrobl-Mazzulla, PH, Moncaut, NP, López, GC, Miranda, LA, Canario, AVM, Somoza, GM
Year of Publication2005
JournalGen Comp Endocrinol
Volume143
Issue1
Date Published2005 Aug
Pagination21-32
ISSN0016-6480
KeywordsAmino Acid Sequence, Animals, Aromatase, Base Sequence, Brain, DNA, Complementary, Female, Fishes, Gene Expression Regulation, Enzymologic, Immunochemistry, Male, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Zebrafish Proteins
Abstract

The brain-type aromatase (CYP19A2) cDNA from pejerrey Odontesthes bonariensis was characterized. Its sequence differs from the ovarian-derived aromatase (CYP19A1) previously reported for the same species. The cDNA is 2305bp in length and the deduced protein comprises 501 amino-acids. The percentage of identity was higher when compared to other brain-derived aromatase proteins (85-63%) and lower with ovarian-derived aromatases (64-57%). Pejerrey aromatases share 61% of identity. The tissue expression analysis showed that CYP19A2 was expressed in the kidney, brain, and pituitary gland of both sexes and also in the ovary, but not in the eye, spleen, liver, gill, and testis. Semi-quantitative RT-PCR analysis of different brain areas revealed that CYP19A2 was expressed significantly higher in anterior male brain areas than in the corresponding female areas, and also when compared to posterior brain areas from both sexes. An immunological analysis using a polyclonal anti-teleost aromatase showed immunoreactive aromatase cells bordering the telencephalic ventricle and a strong signal in the ependymal cells of the preoptic area and the hypothalamus. In the optic tectum immunoreactive aromatase cells were labeled in the ventral wall and in the ependymal layer of the third and fourth ventricle with lateral projections. In the pituitary gland immunoreactive aromatase cells could be found in the rostral and proximal pars distalis. In this gland, aromatase fibers were also detected in different areas; many of them concentrated around blood vessels.

DOI10.1016/j.ygcen.2005.02.026
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/15993101?dopt=Abstract

Alternate JournalGen. Comp. Endocrinol.
PubMed ID15993101
CCMAR Authors