THREE DAY PROTEOMICS COURSE: FROM 2DE TO PROTEIN IDENTIFICATION BY MASS SPECTROMETRY
Proteomics is the study of the entire collection of proteins of a biological sample. Besides all the recent progress in this area of research that aims to get a deeper insight into the cellular network, a challenge is still presented due to the wide diversity of proteins, modifications and also interactions that are always involved. Proteomic techniques in
conjunction with bioinformatics tools can provide researchers new and powerful tolls to unveil these questions and identify new proteins or complexes.
The course is intended for graduate and postgraduate students who wish to learn about basic proteomics and are willing to gather more information on modern proteomic tools. It is a three day course that includes lectures on advanced proteomics techniques as well as hands-on practical work in the laboratory with the state-ofthe-
art equipment and methods. The aim of the course is to provide an in-depth evaluation of proteomics experiments by offering laboratory training in sample preparation for protein and peptide separations (1-2 DE electrophoresis, IEF,
Protein detection, Western blot methods, DIGE; Image analysis; Troubleshooting), mass spectrometric analyses and subsequent computerbased data analysis and protein interpretation and identification.
Graduate students and researchers interested in the field of proteomics. No previous experience in
2DE and mass spectrometry is required.