|Título||Parathyroid hormone-related protein and calcium regulation in vitamin D-deficient sea bream (Sparus auratus).|
|Publication Type||Journal Article|
|Authors||Abbink, W, Hang, XM, Guerreiro, PM, Spanings, FAT, Ross, HA, Canario, AVM, Flik, G|
|Year of Publication||2007|
|Date Published||2007 Jun|
|Palavras-chave||Animal Feed, Animals, Calcitriol, Calcium, Gene Expression Regulation, Gills, Parathyroid Hormone-Related Protein, Pituitary Gland, Receptor, Parathyroid Hormone, Type 1, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Sea Bream, Vitamin D Deficiency|
Gilthead sea bream (Sparus auratus L.) were fed a vitamin D-deficient diet for 22 weeks. Growth rate, whole body mineral pools and calcium balance were determined. Plasma parathyroid hormone-related protein (PTHrP) and calcitriol levels were assessed. Expression of mRNA for pthrp and pth1r was quantified in gills and hypophysis. Fish on vitamin D-deficient diet (D- fish) showed reduced growth and lower calcium turnover (calcium influx, efflux and accumulation rates decreased) and unaltered plasma calcium levels. Plasma calcitriol levels became undetectable, PTHrP levels decreased in the D- fish. In controls, a significant increase in plasma PTHrP level over time was seen, i.e. it increased with body mass. Relationships were found between plasma PTHrP and the whole body pools of calcium, phosphorus and magnesium, indicative of a role for PTHrP in bone development. Expression of pthrp and pth1r mRNA was down-regulated in the hypophysis of D-fish, whereas in gill tissue, pthrp and pth1r mRNA were up-regulated. We conclude that lower pthrp mRNA expression and plasma values in D- fish reflect lower turnover of PTHrP under conditions of hampered growth; up-regulation of pthrp mRNA in gills indicate compensatory paracrine activity of PTHrP during calcitriol deficiency to guarantee well-regulated branchial calcium uptake. This is the first report to document a relation between PTHrP and calcitriol in fish.
|Alternate Journal||J. Endocrinol.|