Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression. | - CCMAR -

Journal Article

TítuloMolecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression.
Publication TypeJournal Article
AuthorsMoutou, KA, Socorro, S, Power, DM, Mamuris, Z, Canario, AVM
Year of Publication2001
JournalComp Biochem Physiol B Biochem Mol Biol
Volume130
Questão1
Date Published2001 Aug
Pagination13-21
ISSN1096-4959
Palavras-chaveActins, Amino Acid Sequence, Animals, Blotting, Northern, Cloning, Molecular, DNA, Complementary, Fishes, Gene Library, Molecular Sequence Data, Muscles, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction, RNA, Sea Bream, Sequence Homology, Amino Acid, Time Factors, Tissue Distribution
Abstract

In the present study, the Sparus aurata alpha-skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream alpha-actin is identical to Fugu alpha-actin-1. The expression of alpha-actin was initiated at the onset of segmentation. In adult fish, alpha-actin is expressed predominantly in white and red muscle.

Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/11470440?dopt=Abstract

Alternate JournalComp. Biochem. Physiol. B, Biochem. Mol. Biol.
PubMed ID11470440