Interactive effects of a high-quality protein diet and high stocking density on the stress response and some innate immune parameters of Senegalese sole Solea senegalensis. | - CCMAR -

Journal Article

TítuloInteractive effects of a high-quality protein diet and high stocking density on the stress response and some innate immune parameters of Senegalese sole Solea senegalensis.
Publication TypeJournal Article
AuthorsCostas, B, Aragão, C, Dias, J, Afonso, A, Conceição, LEC
Year of Publication2013
JournalFish Physiol Biochem
Volume39
Questão5
Date Published2013 Oct
Pagination1141-51
ISSN1573-5168
Palavras-chaveAmino Acids, Analysis of Variance, Animals, Aquaculture, Blood Glucose, Colorimetry, Complement Pathway, Alternative, Crowding, Dietary Proteins, Flatfishes, Hydrocortisone, Immunity, Innate, Lactic Acid, Muramidase, Peroxidases, Radioimmunoassay, Stress, Physiological
Abstract

Amino acids (AA) regulate key metabolic pathways, including some immune responses. Therefore, this study aimed to assess whether an increased availability of dietary AA can mitigate the expected increase in plasma cortisol and metabolites levels due to high stocking density and its subsequent immunosuppression. Senegalese sole (Solea senegalensis) were maintained at low stocking density (LSD; 3.5 kg m(-2)) or high stocking density (HSD; 12 kg m(-2)) for 18 days. Additionally, both treatments were fed a control or a high protein (HP) diet (LSD, LSD HP, HSD and HSD HP). The HP diet slightly increased the levels of digestible indispensable AA, together with tyrosine and cysteine. HSD was effective in inducing a chronic stress response after 18 days of treatment since fish held at HSD presented higher plasma cortisol, glucose and lactate levels. Moreover, this increase in stress indicators translated in a decrease in plasma lysozyme, alternative complement pathway (ACP) and peroxidase activities, suggesting some degree of immunosuppression. Interestingly, while plasma glucose and lactate levels in HSD HP specimens decreased to similar values than LSD fish, plasma lysozyme, ACP and peroxidase activities increased, with even higher values than LSD groups for ACP activity. It is suggested that the HP diet may be used as functional feed since it may represent a metabolic advantage during stressful events and may counteract immunosuppression in sole.

DOI10.1007/s10695-013-9770-1
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/23341074?dopt=Abstract

Alternate JournalFish Physiol. Biochem.
PubMed ID23341074