First study in cryopreserved Crassostrea angulata sperm. | CCMAR
  • ATT: Cetacean Data Exploration with GIS for Data Analysis (NEW DATE)
  • CCMAR é o único parceiro português em dois projetos europeus de Aquacultura
    O Centro de Ciências do Mar (CCMAR) é parceiro em dois grandes projetos, financiados pela União Europeia, através de fundos H2020, o PerformFISH e o MedAID. Ambos os projetos serão desenvolvidos por equipas do CCMAR, durante os próximos anos.
  • SIBIC2018
    VII Congress of the Iberian Society for Ichthyology, Faro, Portugal, from the 12th to the 16th of June, 2018. REGISTER NOW
  • 24st IUPAC International Conference on Physical Organic Chemistry (ICPOC 24)
    Will take place in Portugal, Faro, from 1 to 6 July 2018.
TítuloFirst study in cryopreserved Crassostrea angulata sperm.
Publication TypeJournal Article
Year of Publication2017
AuthorsRiesco, MF, Félix, F, Matias, D, Joaquim, S, Suquet, M, Cabrita, E
JournalGen Comp Endocrinol
Start Page108
Date Published2016 May 7
Type of Articlejournal article

Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a successful sperm cryopreservation protocol for this important species has been developed for the first time. Different internal cryoprotectants (DMSO, ethylene glycol, polyethylene glycol and methanol) at several concentrations (5, 10, 20%), containers (straws vs cryovials) and freezing rates (slow and fast rates) were tested. Cryoprotectant toxicity tests corroborated that this assay did not take into account the following steps of cryopreservation protocol as sperm agglutination. A fast freezing rate of cells diluted in10% DMSO and the use of straws as containers were the best cryopreservation conditions for Portuguese oyster sperm. Finally, fertilization assays confirmed the efficiency of the cryopreservation protocol in oyster sperm. These results demonstrated that different susceptibilities have been detected concerning sperm cryopreservation depending on oyster species or genetic material composition.


Alternate JournalGen. Comp. Endocrinol.
PubMed ID27167499